Journal: Molecular Medicine Reports

Article Title: Phellinus gilvus -derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells

doi: 10.3892/mmr.2019.10896

Figure Lengend Snippet: KEGG pathway analysis of the p53 signaling pathway regulated by PCA. Copyright permission was granted by Kanehisa Laboratories to adapt a KEGG pathway map for this figure. PCA, protocatechualdehyde.

Article Snippet: An initial activation at 95°C for 2 min was followed by an amplification target sequence of 40 cycles of 95°C for 30 sec, 60°C for 30 sec and 72°C for 30 sec. PCR primers were synthesized by Shanghai Shenergy Biocolor BioScience & Technology Co., Ltd. as follows: p53-induced death domain-containing protein 1 (PIDD) forward 5′-GGGAACCAGTTGAACTTGGAC-3′, reverse 5′-CTGAGCCGCAAAAACTCCAC-3′; p21 forward 5′-CCTGGTGATGTCCGAC-CTG-3′, reverse 5′-CCATGAGCGCATCGCAATC-3′; Cyclin E forward 5′-TCT-TCACACAGATGACACAAGC-3′, reverse 5′-CAACAGCAACCTACAACACCC-3′; Bax forward 5′-AGACAGGGGCCTTTTTGCTAC-3′, reverse 5′-AATTCGCCG-GAGACACTCG-3′; Cyclin D forward 5′-TCAAGTGTGACCCGGACTG-3′, reverse 5′-ATGTCCACATCTCGCACGTC-3′; growth arrest and DNA damage-inducible 45 (Gadd45) forward 5′-CCGAAAGGATGGACACGGTG-3′, reverse 5′-TTATCGGGGTCTACGTTGAGC-3′; p53 forward 5′-CACAGCACATGACGG-AGGTC-3′, reverse 5′-TCCTTCCACCCGGATAAGATG-3′; Bcl-2 forward 5′-GCTACCGTCGTGACTTCGC-3′, reverse 5′-CCCCACCGAACTCAAAGAAGG-3′; cell division cycle-associated protein 2 (Cdc-2) forward 5′-AGAAGGTACTTACGG-TGTGGT-3′, reverse 5′-GAGAGATTTCCCGAATTGCAGT-3′; Cyclin B forward 5′-AAGGCCAAGGTCAGTATGGC-3′, reverse 5′-CTTGCCTGTAGCTCTTCGCT-3′; phosphatidylinositol glycan anchor biosynthesis class S (PIGs) forward 5′-TCT-GTCCCCGATTTCCCCC-3′, reverse 5′-AGCTGCTTCAAGACTTCCGC-3′; GAPDH forward 5′-AAGAAGGTGGTGAAGCAGG-3′, reverse 5′-GAAGGTGGA-AGAGTGGGAGT-3′.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Phellinus gilvus -derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells

doi: 10.3892/mmr.2019.10896

Figure Lengend Snippet: mRNA expression analysis of p53-related genes in B16-F10 cells treated with and without 50 µg/ml PCA for 48 h. mRNA expression level of the following genes: (A) PIDD, p21, cyclin E and Bax; and (B) cyclin D, Gadd45, p53, Bcl-2, Cdc-2, cyclin B and PIGs. Density values were normalized to GAPDH. Data are presented as the mean ± SD from three experiments per group. **P<0.01 vs. control. PCA, protocatechualdehyde; Gadd45, growth arrest and DNA damage-inducible 45; PIDD, p53-induced death domain-containing protein 1; Cdc-2, cell division cycle-associated protein 2; PIGs, phosphatidylinositol glycan anchor biosynthesis class S.

Article Snippet: An initial activation at 95°C for 2 min was followed by an amplification target sequence of 40 cycles of 95°C for 30 sec, 60°C for 30 sec and 72°C for 30 sec. PCR primers were synthesized by Shanghai Shenergy Biocolor BioScience & Technology Co., Ltd. as follows: p53-induced death domain-containing protein 1 (PIDD) forward 5′-GGGAACCAGTTGAACTTGGAC-3′, reverse 5′-CTGAGCCGCAAAAACTCCAC-3′; p21 forward 5′-CCTGGTGATGTCCGAC-CTG-3′, reverse 5′-CCATGAGCGCATCGCAATC-3′; Cyclin E forward 5′-TCT-TCACACAGATGACACAAGC-3′, reverse 5′-CAACAGCAACCTACAACACCC-3′; Bax forward 5′-AGACAGGGGCCTTTTTGCTAC-3′, reverse 5′-AATTCGCCG-GAGACACTCG-3′; Cyclin D forward 5′-TCAAGTGTGACCCGGACTG-3′, reverse 5′-ATGTCCACATCTCGCACGTC-3′; growth arrest and DNA damage-inducible 45 (Gadd45) forward 5′-CCGAAAGGATGGACACGGTG-3′, reverse 5′-TTATCGGGGTCTACGTTGAGC-3′; p53 forward 5′-CACAGCACATGACGG-AGGTC-3′, reverse 5′-TCCTTCCACCCGGATAAGATG-3′; Bcl-2 forward 5′-GCTACCGTCGTGACTTCGC-3′, reverse 5′-CCCCACCGAACTCAAAGAAGG-3′; cell division cycle-associated protein 2 (Cdc-2) forward 5′-AGAAGGTACTTACGG-TGTGGT-3′, reverse 5′-GAGAGATTTCCCGAATTGCAGT-3′; Cyclin B forward 5′-AAGGCCAAGGTCAGTATGGC-3′, reverse 5′-CTTGCCTGTAGCTCTTCGCT-3′; phosphatidylinositol glycan anchor biosynthesis class S (PIGs) forward 5′-TCT-GTCCCCGATTTCCCCC-3′, reverse 5′-AGCTGCTTCAAGACTTCCGC-3′; GAPDH forward 5′-AAGAAGGTGGTGAAGCAGG-3′, reverse 5′-GAAGGTGGA-AGAGTGGGAGT-3′.

Techniques: Expressing